中文名称：25-羟基维生素D（25-OHD）绵羊单克隆抗体
英文名称：Sheep Monoclonal Antibodies (SMAs) to 25-hydroxy vitamin D (25-OH D)
货号：SMA5H10
品牌：Bioventix
PRODUCT DATA SHEET
Product Data Sheet: | Sheep Monoclonal Antibodies (SMAs) to: 25-hydroxy vitamin D (25-OH D) |
SMA Covered | 25-OH D2 specific: vitD2.2D10 25-OH D3 specific: vitD3.2F4 25-OH D2/D3 specific: vitD3.5H10 |
Production Description | The assay of vitamin D levels is of increasing interest and is commonly aimed at the 25-hydroxy variants. Such assays can be required to detect both 25-OH D2 and D3 and the availability of antibodies that can recognise both variants is of use in immunoassay design. |
Manufacturing Specification (all SMAs)
Parameter | Specification | Test Method |
Buffer and pH | PBS +0.1% Sodium azide at 7.2-7.4 | pH Meter |
Antibody Concentration | 1-3 mg/mL | A280 (Ec = 1.4) |
Purity and Aggregates | Single Main Peak >90% | Size Exclusion HPLC |
Immunological Activity | ±20% biological activity of frozen reference standard | In house ELISA |
ELISA DATA: Conjugate Reactivities (3-hydroxy chemistry)
VitD2.2D10 | VitD3.2F4 | vitD3.5H10 | |
25-OH vitD2-3-O-BSA | +++ | +/- | +++ |
25-OH vitD3-3-O-BSA | +/- | +++ | +++ |
ELISA DATA: Free Vitamin Binding
VitD2.2D10 | VitD3.2F4 | vitD3.5H10 | |
Vitamin D2 | - | - | - |
Vitamin D3 | - | - | - |
25-OH vitamin D2 | +++ | +/- | +++ |
25-OH vitamin D3 | +/- | +++ | +++ |
Datasheet last revised Jan 2022
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